Rhizobacteria as Biocontrol Agents of Root Rot Disease on Shallot

Nunik Iriyanti Ramadhan, Hadiwiyono Hadiwiyono, Sudadi Sudadi

Abstract


Shallot is a high-economic value commodit, but so far the supply is still lower than the demand. One of the production problem is “moler” disease of shallot (MDS) caused by Fusarium oxysporum f.sp. cepae (FOCe). The aim of this research was to study the potentiality of shallot rhizobacteria (SRB) from various soil ordo to inhibit (MDS). This research was held in the Laboratory of Biology and Soil Health and Greenhouse at UNS. This research was carried out by exploring rhizobacteria of shallot planted on Entisols, Andisols, and Vertisols. Rhizobacteria exploration results were tested for their ability to control Fusarium oxysporum f.sp.cepae (FOCe). Inhibitory ability test of SRB to FOCe was carried out in vitro and on shallot in the greenhouse. The research cused a Completely Randomized Design (CDR) with two factors. The first factor was rhizobacteria combination and the second factor was various soil ordo (Andisols, Entisols, and Vertisols). Each treatment was repeated three times. It was obtained three rhizobacteria isolates from Vertisols (B15: 70%), Andisols (B12:45,55%), and Entisols (B10:46,67%) being the highest inhibition results to FOCe. The combination of rhizobacteria B12 and B10 provided the lowest intensity.

Keywords


rhizobacteria, soil ordo, moler disease, shallot

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References


Ara K et al. 2006. Foot odor due to microbial metabolism and its control. J Microbiol 52(4)357-64.

BPS (Badan Pusat Statistik) 2012. Produksi sayuran di Indonesia, 1997-2012. http://www.bps.go.id. Diakses pada tanggal 26 September 2013.

Chau JF, Bagtzoglou AC, Willig MR 2011. The effect of soil texture on richness and diversity of bacterial communities. J Env Forens 12:333-341.

Djatnika I 2012. Seleksi bakteri antagonis untuk mengendalikan penyakit layu fusarium pada tanaman phalaenopsis. J Hort 22(3):276-284.

Eichorst SA, Breznak JA, Schmidt TM 2007. Isolation and characterization of soil bacteria that define “terriglobis” gen in the phylum “acidobacteria”. J App and Env Microbiol 73(8): 2708-2717.

Fatawi ZD, HS Gutomo, Hadiwiyono 2003. Studi lini dasar terjadinya epidemi penyakit busuk pangkal bawang putih di tawangmangu. Laporan Hasil Penelitian Penelitian Sumber Dana DUE-Like TA.2003. PS. Agronomi. F. Pertanian.UNS. 45 Hal.

Foth HD 1943. Fundamental of soil sciences. Yogyakarta (ID): Gadjah Mada University Press.

Insafitri 2010. Keanekaragaman, keseragaman, dan dominasi, bivalvia di area buangan lumpur lapindo muara suangai porong. J Kelautan 3(1): 54-59.

Notohadiprawiro Tejoyuwono 1988. Tanah dan lingkungan. Direktorat Jenderal Pendidikan Tinggi Departemen Pendidikan dan Kebudayaan.

Samadi Budi, Cahyono Bambang 2005. Bawang merah. Yogyakarta (ID): Kanisius.

Sessitsch A, Reiter B, Berg G 2004. Endophytic bacterial communities of field-grown potatoplants and their plant-growth-promoting and antagonistic abilities. J Microbiol 50:239-249.

Soesanto L 2006. Pengantar pengendalian hayati penyakit tanaman. Jakarta (ID): Rajawali Press.

Weber KA, Achenbach LA, Coates JD 2006. Microorganism pmping iron: anaerobic microbial iron oxidation and reduction. J Nat Rev Microbiol 4: 752-764.

Yuan J, Raza W, Shen Q, Huang Q 2012. Antifungal activity of bacillus amyloliquetaciens NJN-6 volatile compounds against Fusarium oxysporum f.sp. cubense. J App Env Microbiol 78(16): 5942-5944.




DOI: http://dx.doi.org/10.15608%2Fstjssa.v12i1.217

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