Potensi Isolat Mikrobia Asal Andisol Dieng, Jawa Tengah Sebagai Inokulum Pupuk Hayati Pengoksidasi Sulfur

Sudadi Sudadi, Ika Ernawati, Sumarno Sumarno, Widyatmani Sih Dewi, Hery Widijanto

Abstract


Title : Study on The Potency of Soil Microbes Isolated from Andisols of Dieng, Central Java,  as S-Oxidizing Biofertilizer Inoculant. The problem of nutrient deficiences tend to increase, including sulfur deficiency. The use of high purity NPK fertilizers stimulate sulfur deficiency in agricultural land. This problem can be corrected by the use of elemental sulfur accompanied with S-oxidizing biofertilizer. The study aimed to evaluate the potential of sulfur oxidizing microbial isolated from Andisols of Dieng Central Java as S-oxidizing inoculant. Andisols Dieng naturally contains high sulfur that allegedly inhabited many sulfur-oxidizing microbials. The research consisted of two experiments, each arranged in completely randomized design (CRD). Each experiment consist of two factors namely the kind of microbial isolated from Andisols Dieng and incubation time. The first experiment used Czapek-dox+elemental sulfur medium, while the second experiment used vermicompost as medium. The observed variables include soluble-S, bacterial density, biomass of fungi and medium pH. One isolate of bacteria (I1)and three isolates of fungi (F1, F2 and F3) taken from Andisol of Dieng showing their ability to oxidize sulfur in Czapek-dok agar medium. They were used as isolates in the experiment. The result of the first experiment showed that the oxidized sulfur increased with the length of incubation time as indicated by the increase of soluble-S, bacterial cell density or fungi biomass in the medium followed by the decrease of pH. The second experiment showed that the population of bacteria or fungi isolates and soluble-S of vermicompost were increased collaterally with incubation time. The fungi isolate of F2 have the highest potency as inoculant of S-oxidizing biofertilizer as shown by it ability to survive, grows and oxidize S both in Czapek-dox+elemental sulfur liquid medium as well as in vermicompost medium.

Keywords


Andisols Dieng; biofertilizer inoculant; S-oxidizing microbes

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References


Alexander, M. 1976. Introduction to Soil Microbiology Second Edition. Cornell University. America.

Anandham R, R. Sridar, P. Nalayini, S. Poonguzhali, M. Madhaiyan, and Tongmin SA. 2007. Potential for Plant Growth Promotion in Groundnut (Arachis hypogaea L.) cv. ALR-2 by Co-Inoculation of Sulfur-Oxidizing Bacteria and Rhizobium. Microbiological Research, Science Direct 162 (2007) 139-153.

Ashman MR, dan G. Puri. 2002. Essential Soil Science. Blackwell Publishing. Australia.

Barnett, HI. 1960. Illustrated Genera of Imperfect Fungi. Burgess Publishing Company. West Virginia. Page 63-73.

Buckman, HO dan NC. Brady. 1982. Ilmu Tanah. Bhratara Karya Aksara. Jakarta.

Foth, HD. 1991. Dasar-dasar Ilmu Tanah Edisi Ketujuh. Universitas Gadjah mada. Yogyakarta.

Hamdani, D. 2004. Pengaruh Pemberian Sulfur Dan Inokulum Jamur Pengoksidasi Sulfur (Aspergillus Japonicus dan Penicillium nalgiovese) Terhadap Ketersediaan Sulfat Pada Udipsament. Skripsi. PS Ilmu Tanah. Fakultas Pertanian UGM. Yogyakarta.

Joshi, N.R., AD. Agate, and KM. Paknikar. 2000. Polyamine patterns in iron and sulphur oxidizing bacteria isolated from an Indian copper mine indicate requipment of spermidine for growth under acid conditions. Journal of Microbiology and Biotechnology 16 : 631-634.

Lukito, A. 2005. Ketersediaan Sulfat Pada Entisol, Ultisol dan Vertisol yang Diberi Serbuk Belerang dan Inokulum Jamur Pengoksidasi Belerang (Aspergillus japonicus dan Penicillium nalgiovense). Skripsi. FP UGM Yogyakarta.

Mulyani S., AG.Kartasapoetra, Sastroatmodjo S. 1991. Mikrobiologi Tanah. Rieka Cipta. Jakarta.

Oladele, O. 2005. Microbial Diversity : Biogeochemical Cycling of Phosphorus, Sulfur, Metals and Trace Elemen. Blackwell Publishing. Australia.

Rao, NSS. 1994. Mikroorganisme Tanah dan Pertumbuhan Tanaman (Terjemahan : H. Susilo). Jakarta : Universitas Indonesia Press.

Raistrick, H. and J. Ziffer. 1951. Studies in the biochemistry of micro-organisms. The colouring matters of Penicillium nalgiovensis Laxa. Part 1. Nalgiovensin and nalgiolaxin. Isolation, derivatives and partial structures. Biochem J. 49(5): 563–574.

Rosmakam, A. dan NM. Yuwono. 2002. Ilmu Kesuburan Tanah. Kanisius. Yogyakarta.

Sumarno, H. Widijanto, dan Sudadi. 2008. Inokulasi Jamur Pengoksidasi Belerang Pada Pupuk Fosfat Alam untuk Meningkatkan Fosfat dan Sulfat Tersedia Bagi Tanaman. J. Sains Tanah 5(2) Juli 2008:107-112. ISSN : 1412-3606.

Supriyadi dan Sudadi. 2001. Efektivitas Bakteri Pelarut Fosfat Pada Beberapa Macam Bahan Pembawa Inokulum. J. Sains Tanah 1(1) Juli 2001 : 30-36. ISSN 1412-3606.

Stephen D, dan MD. Bresnik. 2003. Intisari Biologi. Hipokrates. Jakarta. Hal. 47-49.

Sudadi, ID. Prijambada, S. Kabirun, A. Maas, dan J. Widada. 2009. Potensi Aspergillus japonicus dan Penicillium nalgiovense pengoksidasi belerang sebagai pelarut fosfat. Prosiding Seminar Nasional Hasil Penelitian Pertanian. Hal. 188-193.

Sudadi. 2012. Rekayasa pupuk biosulfo berbahan batuan fosfat dan belerang yang diperkaya dengan jamur Aspergillus niger dan Penicillium nalgiovensis. Disertasi. F. Pertanian UGM, Yogyakarta. xx + 238 hlm.

Sumarsih, S. 2001. Pertumbuhan Dan Daya Oksidasi Jamur Pengoksidasi Sulfur Pada Medium Dengan Sumber Karbon Glukosa Atau Polimernya. Tesis. PPS Ilmu Tanah UGM. Yogyakarta.

Supriyani, W. 2006. Pengaruh penambahan asam sulfat terhadap pelarutan fosfor (P) dari batuan fosfat oleh Aspergillus niger. Skripsi. Program Studi Ilmu Tanah. FP UGM, Yogyakarta.

Taalab A S, FA. Hellal, and MA. Abou-Seeda. 2008. Influence of Phosphate Fertilizers Enriched with Sulfur on Phosphorus Availability and Corn Yield in Calcareous Soil in Arid Region. Plant Nutrition Dept. National Research Centre, Dokki, Egypt. Ozean Journal of Applied Sciences 1(1), 2008 ISSN 1943-2429.

Vidyalakshmi, Paranthaman R, and Bhakyaraj R. 2009. Sulphur Oxidizing Bacteria and Pulse Nutrition. World Journal of Agricultural Sciences 2009 5 (3): 270-278.

Wahyuningsih, S. 2006. Pelarutan P dari batuan fosfat oleh aktivitas jamur pengoksidasi belerang. Skripsi. Program Studi Ilmu Tanah. FP UGM, Yogyakarta.




DOI: http://dx.doi.org/10.15608%2Fstjssa.v10i1.130

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